The optical properties of gold nanoparticles are strongly dependent on the shape and size of the particles. Gold nanoparticles can be made in a wide range of shapes and geometries such as spherical particles, nanorods, nanoshells, nanostars, and nanocages. Within their surface plasmon resonance (SPR) wavelength region, gold nanoparticles absorb and/or scatter light intensely, and such properties make them excellent optical materials for bioimaging, biomolecular detection, and photothermal therapy. Gold nanoparticle materials have attracted considerable attention due to their unique properties and potential applications as optical probes.

It reveals information complementary to molecular spectroscopic techniques on gold nanorod–protein interactions. This study demonstrates that DLS is a valuable tool for nanorod characterization. The two serum albumin proteins caused nanorod aggregation upon interaction with the nanorods, while the two immunoglobulin proteins formed a stable protein corona on the nanorod surface without causing significant nanorod aggregation. From this study, we found that protein adsorption to gold nanorods is strongly dependent on the aspect ratio of the nanorods, and varies significantly from protein to protein. We examined the interaction between four different proteins, bovine serum albumin, human serum albumin, immunoglobulin G, and immunoglobulin A (IgA) with four gold nanorods that have the same diameter but different aspect ratios. When proteins are adsorbed to the gold nanorods, the average particle size of the nanorods increases and the rotational diffusion-related size distribution peak also changes dramatically. The small size peak is attributed to the rotational diffusion of the nanorods instead of an actual dimension of the nanorods. In the size distribution diagram, gold nanorods with certain aspect ratios exhibit two size distribution peaks, one with an average hydrodynamic diameter at 5–7 nm, and one at 70–80 nm. Here, we report the use of dynamic light scattering (DLS) for gold nanorods characterization and nanorod–protein interaction study. In order to apply gold nanoparticles in the real biological world, we need to have a better understanding of the potential interactions between gold nanoparticle materials and biomolecules in vivo and in vitro. In recent years, there has been considerable interest and research activity in using gold nanoparticle materials for biomedical applications including biomolecular detection, bioimaging, drug delivery, and photothermal therapy.

Dynamic light scattering ppt